What Is the Fluo-4 NW Calcium Assay?


The Fluo-4 NW Calcium Assay is a two-step procedure that uses a low concentration of pyridylmethyl ethylenediamine (TPEN) to pre-treat samples and remove interference from divalent metals. The resulting signal reflects the amount of calcium in the sample and is read with a microplate reader. This method is nonradioactive and enables high-throughput measurements.

How Does The Process Work?

It is a sensitive and rapid assay based on calcium ion concentration in serum. It measures the amount of intracellular calcium, which is an indicator of GPCR activation, ion channel activity, and cell damage and apoptosis. Because GPCRs are important drug targets, they also play an important role in diverse physiological processes. In order to determine whether a particular chemical is inhibiting GPCRs, calcium ion levels are monitored.

The Fluo4-NW Calcium Assay enables researchers to examine the intracellular calcium level of different cell types. The kit’s no-wash protocol reduces the %CVs and ensures the highest quality of data. The assay’s easy-to-use software makes it simple for researchers to test many samples. One of its main features is its non-radioactivity. The assay is sensitive and rapid.

The Fluo4-NW Calcium Assay can be used on a Fluorescence microplate reader with bottom-read mode. The protocol eliminates the presence of pyridylmethyl ethylenediamine, which can interfere with the assay. The Cayman’s Calcium Assay is highly accurate and fast. The results are available in a single hour. It can be used in many different samples and has a wide range of applications.

The CLARIOstar Plus multi-mode plate reader is the ideal instrument for the calcium assay. Its advanced bottom-reading optical system provides ultra-fast data sampling. Its multiple-measurement modes and Atmospheric Control Unit allow for fast and accurate results. The device is equipped with reagent injectors and can accommodate 96-well plates. If you’re planning to perform more than 250 assays, this kit will meet all of your needs.

Applications For The Calcium Assay

A calcium assay is important for many reasons. It can measure cytoplasmic calcium levels in living cells. It can also monitor the effects of different drugs. Aside from helping researchers learn more about the mechanisms of drug action, it can also be used for assessing the effect of a chemical on a cell. In this way, the fluorescence in living cells can be easily measured in laboratory environments. The use of fluorescent dyes in the assays can make it easier to find a specific substance.

The FluoFORTE Calcium Assay Kits measure intracellular calcium mobilization. A calcium assay uses a unique, fluorescent calcium-binding dye designed to be highly permeability and superior binding sensitivity. With the FLUOFORTE Calcium Fluorometric Assay kit, you can easily measure intracellular calcium levels in biological fluids such as blood, urine, and saliva. A low concentration of calcium may cause a toxicity or health condition.

Effective Research And Learning

Based on the information that we have discussed, it is important to understand the impact that research and learning can have on the functions of the Calcium assay and how effectively it works overall. The calcium assay is helping to advance and grow research into cardiac functions amongst other health conditions. Data and information should be shared as much as possible in order for the wider medical community to gain a better understanding of a variety of different kinds of health conditions.